ENDOSAFE PTS PDF

The Endosafe®-PTS™ is a handheld spectrophotometer that utilizes FDA- licensed disposable cartridges for accurate, convenient endotoxin testing. With test. A multicenter comparison study between the Endosafe PTS rapid-release testing system and traditional methods for detecting endotoxin in cell-therapy products. The Endosafe®-PTS™ is a rapid, point-of-use test system that provides quantitative LAL results within 15 minutes. The PTS™ utilizes LAL reagents in an .

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Endosafe-PTS Glucan Assay from Charles River

Preliminary results with these cartridges showed similar patterns to those observed for endotoxin, but glucan was found at higher levels and found to be far more widespread throughout the ISS. By using this site, you agree to the Terms of Use and Privacy Policy.

Valid paired results, based on each center’s LAL method and the Endosafe system criteria, were analyzed. The Endosafe system provided reliable results with products typically produced in cell-therapy manufacturing facilities, and would be an appropriate test on which to base the release of time-sensitive cell-therapy products. An astronaut can take a swab sample from a cabin surface, dissolve it in endotoxin-free water, and analyze it quantitatively with LOCAD-PTS in microgravity, all in under 15 minutes.

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The projects develop integrated lab-on-a-chip products in three areas related to space exploration: Tests with these cartridges have been performed during Expeditions 18 — All samples tested with the Ebdosafe system used 0.

In other projects Wikimedia Commons. This ‘swab’ was performed indirectly with the spacesuit gloves. Subsequently, each center tested the endotoxin content of standard dilutions of cell-therapy products, thus creating paired endosfae results for each sample. Gravitational Physiology, Volume 10, Issue 2, This page was last edited on 8 Novemberat Additionally, fabricated endotoxin-positive samples containing varying concentrations of endotoxin were prepared and shipped to all centers to perform blinded testing.

Archived from ptss original on The goal of the experiment is to design and test procedures to monitor and document the spread endosfae biological material associated with human spaceflight, both before and after launch, in preparation for the human exploration of the Moon and Mars.

These three molecules are found mainly within gram-negative bacteriafungi and gram-positive bacteriarespectively. Retrieved from ” https: Endotoxin detection between paired results was equivalent in most cases.

Karissa West performs microbial survey of Pad 39A August 7, From Wikipedia, the free encyclopedia. Views Read Edit View history.

Endosafe nexgen-PTS from Charles River – Product Description and Details

Before LOCAD-PTS was launched to space, it was tested extensively in simulated microgravity with the use of parabolic flightto verify nominal function of the instrument and sample handling procedures. Endotoxin and glucan are detected with the Limulus amebocyte lysate LAL enzyme cascade, derived from the horseshoe crab Limulus polyphemus.

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Current assay systems are labor intensive and time consuming. Archived copy as title Commons category link is on Wikidata.

Charles River Endosafe PTS Endotoxin Testing System

By the amplification of a small signal, these enzyme cascades allow detection of very low amounts of starting microbial material. The Endosafe portable test system PTS is a chromogenic Limulus amebocyte lysate LAL portable endotoxin detection system that provides quantitative results in approximately 15 min.

The device itself or reader weighs 2. Rapid-release testing reduces the waiting period for administration of endksafe cell-therapy products.

Endotoxin was found at every surface site where Colony Forming Units CFUs were observed with culture-based contact slide methods. Traditional culture-based methods aboard the ISS take several days and often require return of samples to Earth for more in-depth analysis by scientists on the ground.