Redei, G.P. () Supervital mutants of Arabidopsis. Genetics, 47 Simpson, G.G. &Dean, C. () Arabidopsis, the Rosetta stone of flowering time?Science . casein protein kinase 2 alphasubunit genes. Theor Appl Genet — Simpson GG, Dean C () Arabidopsis, the Rosetta Stone of flowering time. [CrossRef] [PubMed] Simpson, G.G.; Dean, C. Arabidopsis, the Rosetta stone of flowering time? Science , , – [CrossRef] [PubMed] Valentin, F.L. .
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GUS plants, indicating CO is arabidopsks in vascular tissues. These results suggest that despite its structural homology to HP1, TFL2 mainly functions as a repressor of both specific euchromatin genes such as FT and several floral homeotic genes in Arabidopsis.
A From left to right, day-old tfltfl;ft-1ft-1 and WT Col plants. Multiple roles of Arabidopsis VRN1 in vernalization and flowering time control. Chronobiol Int 20, This drastic difference in arabieopsis patterns enables us to assess the effects of genetic modification on the photoperiodic response more precisely.
Ann Bot Nat Rev Mol Cell Biol 4, Citing articles via Web of Science A TFL2 complements the swi6 — mutant in fission yeast. Abstract Floral transition should be strictly regulated because it is one of the most critical developmental processes in plants.
This framework provides the basis to understand the evolution of different reproductive strategies and how floral pathways interact through seasonal progression. FT protein is a major part of florigen a long-sought flowering-inducing substance and it is synthesized in the leaf vasculature . The other question that should be addressed in future studies is how the ubiquitously expressed TFL2 gene can repress target genes in the inactivated phase and derepress in the activated phase of the plant.
The ectopic expression of these genes may be related to the curled leaf phenotype of tlf2because constitutive expression of SEP3AG and both PI and AP3 is known to lead to a curled leaf morphology Mizukami and MaKrizek and MeyerowitzHonma and Goto We isolated the genomic DNA of tfl Five and four optical sections were projected, respectively. The need for winter in the switch to flowering. We used the aerial parts of seedlings without leaves leaves were cut off at petioles or rosette leaves.
The difference between LD and SD was more obvious when the plants were grown on plates; that is, while the increase was EMBO J 27, In transgenic Arabidopsis carrying a TFL2: From This Paper Figures, tables, and topics from this paper.
Curr Opin Plant Biol 15, We carried out the microarray analysis using an Affymetrix GeneChip containing about Arabidopsis genes first version.
Arabidopsis, the Rosetta stone of flowering time?
TFL2 represents such a protein, and these findings suggest that early-flowering mutants provide a good tool with which to identify repressors that are important in the flowering pathway. University of Washington, Department of Biology. Plant Cell 17, Epigenetic Regulation in the Control of Flowering J.
Search for related content. In contrast to the clear repression of these euchromatin genes, the expression of heterochromatin genes is not released from silencing in the tfl2 mutant.
The day-length-insensitive early-flowering phenotype of tfl2 indicated that the TFL2 gene might function in the photoperiod pathway. Plant crossing was carried out by manual cross-pollination. A repressor of flowering in Arabidopsis regulated by vernalization and methylation. We first focused on the early-flowering phenotype of tfl2 rime, which is relatively insensitive to day length, because this phenotype suggests that TFL2 gene may function to repress target gene s involved in the photoperiod pathway.
Arabidopsis, the Rosetta stone of flowering time?
The major genetic pathways that control the transition can currently be characterized as photoperiodic, vernalization, autonomous, and gibberellin [10,21,22]. For wild type, error bars lie within circles.
To accurately measure differences in day length, circadian clock-dependent timing regulation of CO is a crucial mechanism [35,36]. Timme of 3 references. Arabidopsis mutants showing an altered response to vernalization. GI is a large nuclear protein without known functional domain , and FKF1 is a LOV domain containing a blue-light photoreceptor that regulates protein degradation [43,44].
Epigenetic Regulation in the Control of Flowering
References Publications referenced by this paper. Arabidopsis terminal flower 2 tfl2 mutants show an early-flowering arabudopsis that is relatively insensitive to photoperiod, as well as several other pleiotropic phenotypes. Plant Cell 20, For the flowering time and for other phenotypes, we did not find any difference in the severity among three different alleles of tfl2 Table 1.
Cell walls were stained by propidium iodide and visualized by the same excitation with a long-pass filter ELP. Days are relative to sowing, which is designated as day 0. SimpsonCaroline Dean Published in Science Multiple environmental and endogenous inputs regulate when plants flower.