51116 DATASHEET PDF

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Fragile X syndrome is a genetic disorder characterized by a spectrum of physical and behavioral features and is a frequent form of inherited mental retardation 1.

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Antibodies are purified by protein Datasheett and peptide affinity chromatography. Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Application Dilutions Western Blotting 1: Treat cells by adding fresh media containing regulator for desired time. Prepare solutions with reverse osmosis deionized RODI or equivalently purified water.

This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody. These results suggest that fragile X syndrome is related to abnormal translation caused by defects in RNAi-related pathways. Blotting Membrane and Paper: Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment.

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Would you like to visit your country specific website? Proceed with detection Datashee D. Aspirate media from cultures; wash cells with 1X PBS; aspirate.

Volumes are for 10 cm x 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly. From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Protein Blotting A general protocol for sample preparation.

Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. More about how we get our images.

(PDF) TPS51116 Datasheet download

It should be noted that for the best possible results a fresh blot is always recommended. Wash three times for 5 min each with 15 ml of TBST. Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature. Prepare solutions with reverse osmosis deionized RODI or equivalent grade water.

Do not aliquot the antibody. FXR2 Antibody – Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available.

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Microcentrifuge for 5 min. Please refer to primary antibody datasheet or product webpage for recommended antibody dilution. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Detection of Proteins Directions for Use: Each of the fragile X proteins can self-associate, as well as form heteromers with the other two related proteins 3.

Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in plastic and expose to X-ray film. Find answers on our FAQs page. Changing to another country datwsheet result in loss of shopping cart. Biotinylated Protein Ladder Detection Pack: Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity.

Electrotransfer to nitrocellulose membrane Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human FXR2 protein.

To Purchase S View sizes. Dilute to 1X with dH 2 O. Primary Antibody Dilution Buffer: